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Crystal structures of wild-type AdiC in the presence and absence of the substrate agmatine at 2.6-Å and 2.2-Å resolution.

Please see our recent publication: Ilgü et al. (2016), Proc. Natl. Acad. Sci. USA

Eins->Zwei->Drei

Publications in 2017

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84. Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change. 
Kalbermatter D., Chiu P.L., Jeckelmann J.-M., Ucurum Z., Walz T. and Fotiadis D. J. Struct. Biol. (2017) in press.

83. Detecting ligand-binding events and free energy landscape while imaging membrane receptors at subnanometer resolution. 
Pfreundschuh M., Harder D., Ucurum Z., Fotiadis D. and Müller D.J. Nano Lett. (2017) in press.

82. Sensory rhodopsin I and sensory rhodopsin II form trimers of dimers in complex with their cognate transducers. 
Orekhov P., Bothe A., Steinhoff H.-J., Shaitan K.V., Raunser S., Fotiadis D., Schlesinger R., Klare J.P. and Engelhard M. Photochem. Photobiol. (2017) in press.

Publications in 2016

81. Insights into the molecular basis for substrate binding and specificity of the wild-type L-arginine/agmatine antiporter AdiC. 
Ilgü H., Jeckelmann J.-M., Gapsys V., Ucurum Z., de Groot B.L. and Fotiadis D. Proc. Natl. Acad. Sci. USA (2016) 113, 10358-10363.

80. Engineering a chemical switch into the light-driven proton pump proteorhodopsin by cysteine mutagenesis and thiol modification. 
Harder D., Hirschi S., Ucurum Z., Goers R., Meier W., Müller D.J. and Fotiadis D. Angew. Chem. Int. Ed. Engl. (2016) 55, 8846-8849.

79. Engineering and assembly of protein modules into functional molecular systems.  
Hirschi S., Stauffer M., Harder D., Müller D.J., Meier W. and Fotiadis D. Chimia (2016) 70, 398-401.

78. Purification of human and mammalian membrane proteins expressed in Xenopus laevis frog oocytes for structural studies.
Boggavarapu R., Hirschi S., Harder D., Meury M., Ucurum Z., Bergeron M.J. and Fotiadis D. Methods Mol. Biol. (2016) 1432, 223-242.

Publications in 2015

77. 2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli.
Kalbermatter D., Jeckelmann J.-M., Chiu P.-L., Ucurum Z., Walz T. and Fotiadis D. J. Struct. Biol. (2015) 191, 376-380.

76. Role of electrostatic interactions for ligand recognition and specificity of peptide transporters.
Boggavarapu R., Jeckelmann J.-M., Harder D., Ucurum Z. and Fotiadis D. BMC Biology (2015) 13, 58.

75. Cultivation strategies to enhance productivity of Pichia pastoris: A review.
Looser V., Brühlmann B., Bumbak F., Stenger C., Costa M., Camattari A., Fotiadis D. and Kovar K. Biotechnol Adv. (2015) 33, 1177-1193.

74. Single-molecule force spectroscopy of membrane proteins from membranes freely spanning across nanoscopic pores.
Petrosyan R., Bippes C., Walheim S., Harder D., Fotiadis D., Schimmel T., Alsteens D. and Müller D.J. Nano Lett. (2015) 15, 3624-3633.

73. Two-dimensional crystallization of membrane proteins and structural assessment.
Fotiadis D. and Engel A. eLS (2015) DOI: 10.1002/9780470015902.a0003041.pub2.

72. High-resolution atomic force microscopy imaging of rhodopsin in rod outer segment disk membranes.
Bosshart P., Engel A. and Fotiadis D. Methods Mol. Biol. (2015) 1271, 189-203.

Publications in 2014

71.  Detergent-induced stabilization and improved 3D map of the human heteromeric amino acid transporter 4F2hc-LAT2.
Meury M., Costa M., Harder D., Stauffer M., Jeckelmann J.-M., Brühlmann B., Rosell A., Ilgü H., Kovar K., Palacin M. and Fotiadis D. PLoS ONE (2014) 9, e109882.

70. 2D and 3D crystallization of a bacterial homologue of human vitamin C membrane transport proteins.
Jeckelmann J.-M., Harder D., Ucurum Z. and Fotiadis D. J. Struct. Biol. (2014) 188, 87-91.

69. Structural bases for the interaction and stabilization of the human amino acid transporter LAT2 with its ancillary protein 4F2hc.
Rosell A., Meury M., Alvarez-Marimon E., Costa M., Perez-Cano L., Zorzano A., Fernadez-Recio J., Palacin M. and Fotiadis D. Proc. Natl. Acad. Sci. USA (2014) 111, 2966-2971.

68. Variation of the detergent binding capacity and phospholipid content of membrane proteins when purified in different detergents.
Ilgü H., Jeckelmann J.-M., Gachet S.M., Boggavarapu R., Ucurum Z., Gertsch J. and Fotiadis D. Biophys. J. (2014) 106, 1660-1670.

Publications in 2013

67. Peptide transporter DtpA has two alternate conformations, one of which is promoted by inhibitor binding.
Bippes C., Ge L., Meury M., Harder D., Ucurum Z., Daniel H., Fotiadis D. and Müller D.J. Proc. Natl. Acad. Sci. USA (2013) 110, E3978-E3986.

D.J.M. and D.F. share senior authorship and correspondence.

66. Expression, purification and low-resolution structure of human vitamin C transporter SVCT1 (SLC23A1).
Boggavarapu R., Jeckelmann J.-M., Harder D., Schneider P., Ucurum Z., Hediger M. and Fotiadis D. PLoS ONE (2013) 8, e76427.

65. The small SLC43 family: facilitator system L amino acid transporters and the orphan EEG1.
Bodoy S., Fotiadis D., Stöger C., Kanai Y. and Palacin M. Mol. Aspects Med. (2013) 34, 638-645.

64. The SLC3 and SLC7 families of amino acid transporters.
Fotiadis D., Kanai Y. and Palacin M. Mol. Aspects Med. (2013) 34, 139-158.

63. Crystal clear.
Fotiadis D. International Innovation (2013) Special Issue: Health Partnership January 2013, 103-105.

62. High-resolution imaging of 2D outer membrane protein F (OmpF) crystals by atomic force microscopy.
Fotiadis D. and Müller D.J. Methods Mol. Biol. (2013) 736, 461-474.

61. Expression of human heteromeric amino acid transporters in the yeast Pichia pastoris.
Costa M., Rosell A., Alvarez-Marimon E., Zorzano A., Fotiadis D. and Palacin M. Protein Expr. Purif. (2013) 87, 35-40.

M.P. and D.F. share senior authorship and correspondence.

Publications in 2012

60. Membrane proteins - at the core of life.
Kuhn A. and Fotiadis D. Public Service Review: European Science and Technology (2012) 17, 92-93.

59. Measuring substrate binding and affinity of purified membrane transport proteins using the scintillation proximity assay.
Harder D. and Fotiadis D. Nature Protocols (2012) 7, 1569-1578.

58. Membrane disrupting antimicrobial peptide dendrimers with multiple amino termini.
Stach M., Maillard N., Kadam R.U., Kalbermatter D., Meury M., Page M.G.P., Fotiadis D., Darbre T. and Reymond J.-L. Med. Chem. Commun. (2012) 3, 86-89.

57. Atomic force microscopy for the study of membrane proteins.
Fotiadis D. Curr. Opin. Biotechnol. (2012) 23, 510-515.

Publications in 2011

56. Structure and function of the glucose PTS transporter from Escherichia coli.
Jeckelmann J.-M., Harder D., Mari S.A., Meury M., Ucurum Z., Müller D.J., Erni B. and Fotiadis D. J. Struct. Biol. (2011) 176, 395-403.

55. Frog oocytes to unveil the structure and supramolecular organization of human transport proteins.
Bergeron M.J., Boggavarapu R., Meury M., Ucurum Z., Caron L., Isenring P., Hediger M. and Fotiadis D. PLoS ONE (2011) 6, e21901.

54. Structure determination of channel and transport proteins by high-resolution microscopy techniques.
Meury M., Harder D., Ucurum Z., Boggavarapu R., Jeckelmann J.-M. and Fotiadis D. Biol. Chem. (2011) 392, 143-150.

53. A tool for the qualitative comparison of membrane-embedded and detergent-solubilized membrane protein structures in projection.
Jeckelmann J.-M., Palacin M. and Fotiadis D. J. Struct. Biol. (2011) 173, 375-381.

Publications in 2009

52. Projection structure of DtpD (YbgH), a prokaryotic member of the peptide transporter family.
Casagrande F., Harder D., Schenk A.D., Meury M., Ucurum Z., Engel A., Weitz D., Daniel H. and Fotiadis D. J. Mol. Biol. (2009) 394, 708-717.

51. Substrate binding tunes confromational flexibility and kinetic stability of an amino acid antiporter.
Bippes C.A., Zeltina A., Casagrande F., Ratera M., Palacin M., Müller D.J. and Fotiadis D. J. Biol. Chem. (2009) 284, 18651-18663.

50. Isolation and functional characterization of a stable complex between photoactivated rhodopsin and the G protein, transducin.
Jastrzebska B., Golczak M., Fotiadis D., Engel A. and Palczewski K. FASEB J. (2009) 23, 371-381.

Publications in 2008

49. Projection structure of a member of the amino acid/polyamine/organocation transporter superfamily.
Casagrande F., Ratera M., Schenk A. D., Chami M., Valencia E., Lopez J.M., Torrents D., Engel A., Palacin M., Fotiadis D. J. Biol. Chem. (2008) 283, 33240-33248.

48. Potential of interferometric cantilever detection and its application for SFM/AFM in liquids.
Hoogenboom B.W., Frederix P.L.T.M., Fotiadis D., Hug H.J. and Engel A. Nanotechnology (2008) 19, 384019 (6pp).

47. High-throughput single-molecule force spectroscopy for membrane proteins.
Bosshart P.D., Casagrande F., Frederix P.L.T.M., Ratera M., Bippes C.A., Müller D.J., Palacin M., Engel A. and Fotiadis D. Nanotechnology (2008) 19, 384014 (15pp).

46. DtpB (YhiP) and DtpA (TppB, YdgR) are prototypical proton-dependent peptide transporters of Escherichia coli.
Harder D., Stolz J., Casagrande F., Obrdlik P., Weitz D., Fotiadis D. and Daniel H. FEBS J. (2008) 275, 3290-3298.

45. Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer.
Whorton M.R., Jastrzebska B., Park P.S.-H., Fotiadis D., Engel A., Palczewski K. and Sunahara R. J. Biol. Chem. (2008) 283, 4387-4394.

44. Single-molecule microscopy and force spectroscopy of membrane proteins.
Engel A., Janovjak H., Fotiadis D., Kedrov A., Cisneros D. and Müller D.J. In Single Molecules and Nanotechnology (Rigler R. and Vogel H., eds.), Springer Verlag, Berlin, Germany (2008).

Publications in 2007

43. Reconstitution of mitochondrial ATP synthase into lipid bilayers for structural analysis.
Arechaga I. and Fotiadis D. J. Struct. Biol. (2007) 160, 287-294.

42. The supramolecular assemblies of voltage-dependent anion channels in the native membrane.
Hoogenboom B.W., Suda K., Engel A. and Fotiadis D. J. Mol. Biol. (2007) 370, 246-255.

41. Functional and structural characterization of the first prokaryotic member of the LAT family: a model for APC transporters.
Reig N., del Rio C., Casagrande F., Ratera M., Gelpi J.L., Torrents D., Henderson P.J., Baldwin S.A., Zorzano A., Fotiadis D. and Palacin M. J. Biol. Chem. (2007) 282, 13270-13281.

M.P. and D.F. share senior authorship and correspondence.

40. Functional and structural characterization of a prokaryotic peptide transporter with similar features as mammalian PEPT1.
Weitz D., Harder D., Casagrande F., Fotiadis D., Oberdlik P., Kelety B. and Daniel H. J. Biol. Chem. (2007) 282, 2832-2839.

Publications in 2006

39. Bacteriorhodopsin chimeras containing the third cytoplasmic loop of bovine rhodopsin activate transducin for GTP/GDP exchange.
Geiser A.H., Sievert M.K., Guo L.W., Grant J.E., Krebs M.P., Fotiadis D., Engel A. and Ruoho A.E. Protein Sci. (2006) 7, 1679-1690.

38. Quantitative dynamic-mode scanning force microscopy in liquid.
Hoogenboom B.W., Hug H.J., Pellmont Y., Martin S., Frederix P.L.T.M., Fotiadis D. and Engel A. Appl. Phys. Lett. (2006) 88, 193109.

37. Functional and structural characterization of rhodopsin oligomers.
Jastrzebska B., Fotiadis D., Jang G.F., Stenkamp R.E., Engel A. and Palczewski K. J. Biol. Chem. (2006) 281, 11917-11922.

36. Assessing the structure and dynamics of native membrane proteins.
Kukulski W., Kaufmann T., Braun T., Rémigy H.-W., Fotiadis D. and Engel A. In Structural Biology of Membrane Proteins (Grisshammer R. and Buchanan S.K., eds.), Royal Society of Chemistry, London, United Kingdom (2006).

35. Single-molecule studies of membrane proteins.
Müller D.J., Sapra K.T., Scheuring S., Kedrov A., Frederix P.L.T.M., Fotiadis D. and Engel A. Curr. Opin. Struct. Biol. (2006) 16, 489-495.

34. Structure of the rhodopsin dimer: a working model for G-protein-coupled receptors.
Fotiadis D., Jastrzebska B., Philippsen A., Müller D.J., Palczewski K. and Engel A. Curr. Opin. Struct. Biol. (2006) 16, 252-259.

33. Atomic force microscopy in biology.
Fotiadis D., Frederix P.L.T.M. and Engel A. In Cell Biology: A Laboratory Handbook (Celis J.E., ed.), Academic Press, New York, NY, USA (2006).

Publications in 2005

32. The 5 Å structure of heterologously expressed plant aquaporin SoPIP2;1.
Kukulski W., Schenk A.D., Johanson U., Braun T., de Groot B.L., Fotiadis D., Kjellbom P. and Engel A. J. Mol. Biol. (2005) 350, 611-616.

Publications in 2004

31. Electron and atomic force microscopy of the trimeric ammonium transporter AmtB.
Conroy M.J., Jamieson S.J., Blakey D., Kaufmann T., Engel A., Fotiadis D., Merrick M. and Bullough P.A. EMBO Reports (2004) 5, 1153-1158.

30. Functional characterization of rhodopsin monomers and dimers in detergents.
Jastrzebska B., Maeda T., Zhu L., Fotiadis D., Filipek S., Engel A., Stenkamp R.E. and Palczewski K. J. Biol. Chem. (2004) 279, 54663-54675.

29. The supramolecular structure of the GPCR rhodopsin in solution and native disc membranes.
Suda K., Filipek S., Palczewski K., Engel A. and Fotiadis D. Mol. Mem. Biol. (2004) 21, 435-446.

28. Rhodopsin signaling and organization in heterozygote rhodopsin knockout mice.
Liang Y., Fotiadis D., Maeda T., Maeda A., Modzelewska A., Filipek S., Saperstein D.A., Engel A. and Palczewski K. J. Biol. Chem. (2004) 279, 48189-48196.

27. A concept for G protein activation by G protein-coupled receptor dimers: the transducin/rhodopsin interface.
Filipek S., Krzysko K.A., Fotiadis D., Liang Y., Saperstein D.A., Engel A. and Palczewski K. Photochemical & Photobiological Sciences (2004) 3, 628-638.

26. The G protein-coupled receptor rhodopsin in the native membrane.
Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A. and Palczewski K. FEBS Letters (2004) 564, 281-288.

25. Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX.
Siebert C.A., Qian P., Fotiadis D., Engel A., Hunter C.N. and Bullough P.A. EMBO J. (2004) 23, 690-700.

24. Structural analysis of the RC-LH1 photosynthetic core complex of Rhodospirillum rubrum using atomic force microscopy.
Fotiadis D., Qian P., Philippsen A., Bullough P.A., Engel A. and Hunter C.N. J. Biol. Chem. (2004) 279, 2063-2068.

23. High resolution imaging of bacteriorhodopsin by atomic force microscopy.
Fotiadis D. and Engel A. In Atomic Force Microscopy: Methods and Applications (Braga P.C. & Ricci D., eds.). Humana Press Inc., Totowa, NJ, USA (2004).

Publications in 2003

21. Determining molecular forces that stabilize human aquaporin-1.
Möller C., Fotiadis D., Suda K., Engel A., Kessler M. and Müller D.J. J. Struct. Biol. (2003) 142, 369-378.

20. Organization of the G protein-coupled receptor rhodopsin and opsin in native membranes.
Liang Y., Fotiadis D., Filipek S., Saperstein D.A., Palczewski K. and Engel A. J. Biol. Chem. (2003) 278, 21655-21662.

L.Y. and D.F. share first authorship.

19. Reconstitution of water channel function of an aquaporin overexpressed and purified from Pichia pastoris.
Karlsson M., Fotiadis D., Sjövall S., Johansson I., Hedfalk K., Engel A. and Kjellbom P. FEBS Letters (2003) 537, 68-72.

18. Atomic-force microscopy: Rhodopsin dimers in native disc membranes.
Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A. and Palczewski K. Nature (2003) 421, 127-128.

17. Atomic force bio-analytics.
Frederix P.L.T.M., Akiyama T., Staufer U., Gerber C., Fotiadis D., Müller D.J. and Engel A. Curr. Opin. Chem. Biol. (2003) 7, 641-647.

16. Biophysics (communication arising): Is rhodopsin dimeric in native retinal rods?
Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A. and Palczewski K. Nature (2003) 426, 31.

Publications in 2002

15. Identification and structure of a putative Ca2+-binding domain at the C terminus of AQP1.
Fotiadis D., Suda K., Tittmann P., Jenö P., Philippsen A., Müller D.J., Gross H. and Engel A. J. Mol. Biol. (2002) 318, 1381-1394.

14. Progress in analysis of membrane protein structure and function.
Werten P., Rémigy H.-W., de Groot B., Fotiadis D., Philippsen A., Stahlberg H., Grubmüller H. and Engel A. FEBS Letters (2002) 529, 65-72.

Publications in 2001

13. Bacterial Na+-ATP synthase has an undecameric rotor.
Stahlberg H., Müller D.J., Suda K., Fotiadis D., Engel A., Meier T., Matthey U. and Dimroth P. EMBO Reports (2001) 2, 229-233.

12. Structural characterization of two aquaporins isolated from native spinach leaf plasma membranes.
Fotiadis D., Jenö P., Mini T., Wirtz S., Müller S.A., Fraysse L., Kjellbom P. and Engel A. J. Biol. Chem. (2001) 276, 1707-1714.

11. Single proteins observed by atomic force microscopy.
Scheuring S., Fotiadis D., Möller C., Müller S.A., Engel A. and Müller D.J. Single Molecules (2001) 2, 59-67.

10. Imaging and manipulation of biological structures with the AFM.
Fotiadis D., Scheuring S., Müller S.A., Engel A. and Müller D.J. Micron (2001) 33, 385-397.

9. Two-dimensional crystals: A powerful approach to assess structure, function and dynamics of membrane proteins.
Stahlberg H., Fotiadis D., Scheuring S., Rémigy H.-W., Braun T., Mitsuoka K., Fujiyoshi Y. and Engel A. FEBS Letters (2001) 504, 166-172.

Publications in 2000

8. Surface tongue-and-groove contours on lens MIP facilitate cell-to-cell adherence.
Fotiadis D., Hasler L., Müller D.J., Stahlberg H., Kistler J. and Engel A. J. Mol. Biol. (2000) 300, 779-789.

7. Conformations of the rhodopsin third cytoplasmic loop grafted onto bacteriorhodopsin.
Heymann J.B., Pfeiffer M., Hildebrandt V., Kaback H.R., Fotiadis D., de Groot B., Engel A., Oesterhelt D. and Müller D.J. Structure (2000) 8, 643-653.

6. Conformational changes, flexibilities and intramolecular forces observed on individual proteins using AFM.
Müller D.J., Fotiadis D., Scheuring S., Möller C. and Engel A. Single Molecules (2000) 1, 115-118.

5. Biochemical and structural analyses of membrane proteins in plants and animals. 
Fotiadis D. Ph.D. thesis (2000). University of Basel, Basel, Switzerland.

Publications in 1999

4. The reaction center complex from the green sulfur bacterium Chlorobium tepidum: A structural analysis by scanning transmission electron microscopy.
Rémigy H.-W., Stahlberg H., Fotiadis D., Müller S.A., Wolpensinger B., Engel A., Hauska G. and Tsiotis G. J. Mol. Biol. (1999) 290, 851-858.

3. Electrostatically balanced subnanometer imaging of biological specimens by atomic force microscope.
Müller D.J., Fotiadis D., Scheuring S., Müller S.A. and Engel A. Biophys. J. (1999) 76, 1101-1111.

Publications in 1998

2. Surface analysis of the photosystem I complex by electron and atomic force microscopy.
Fotiadis D., Müller D.J., Tsiotis G., Hasler L., Tittmann P., Mini T., Jenö P., Gross H. and Engel A. J. Mol. Biol. (1998) 283, 83-94.

1. Mapping flexible protein domains at subnanometer resolution with the atomic force microscope.
Müller D.J., Fotiadis D. and Engel A. FEBS Letters (1998) 430, 105-111.